Expresión del receptor a prolactina en el ganglio pélvico mayor en ratas macho
Resumen
El Ganglio Pélvico Mayor (GPM) forma parte del sistema nervioso periférico, inerva a diversos órganos del área pélvica incluyendo la próstata y se ha mostrado que contiene receptores a hormonas esteroides y a prolactina (PRL). En trabajos anteriores se mostró que ni la ejecución de la conducta sexual ni la denervación preganglionar cambian la expresión del receptor a PRL (RPRL) en GPM, aunque en condiciones de hiperprolactinemia altera su morfología e histología. La PRL ejerce sus funciones mediante su receptor del cual se han reportado al menos tres isoformas que difieren en su dominio intracelular, conocidas como larga, intermedia y corta; sin embargo, en el GPM no se ha evidenciado si existen isoformas para este receptor. El objetivo de este trabajo fue determinar la localización del RPRL en el GPM y la expresión de las diferentes isoformas. Para ello, se extrajo el GPM de ratas macho adultas y se analizó el RPRL mediante inmunodetección en fase sólida e inmunohistoquímica. Los resultados mostraron que, mediante inmunohistoquímica, el RPRL está presente en la membrana de las neuronas del GPM. Mientras que la inmunodetección en fase sólida permitió evidenciar una banda con movilidad relativa de 75 kDa y otra similar a 100 kDa. Esto sugiere la existencia de dos isoformas en el GPM que probablemente correspondan a las isoformas reportadas corta y larga. Concluimos que las neuronas del GPM expresan el RPRL y que los efectos de PRL son mediados posiblemente por estas dos isoformas.
Abstract
The Major Pelvic Ganglion (MPG) is part of the peripheral nervous system, innervates several organs of the pelvic area including the prostate, and it has been shown to contain receptors for steroid hormones and prolactin (PRL). In previous works, it was shown that neither the execution of sexual behavior nor preganglionic denervation changes the expression of the prolactin receptor (RPRL) in the MPG, but in conditions of hyperprolactinemia, its morphology and histology are modified. Prolactin exerts its functions through its receptor with at least three isoforms that differ in the intracellular domain known as long, intermediate, and short. However, in the MPG it has not been shown whether isoforms exist for this receptor. This work aimed to determine the RPRL location in the MPG tissue and the existence of the different isoforms. The MPG was extracted from adult male rats, and the prolactin receptor was analyzed using solid-phase immunodetection and immunohistochemistry. Immunohistochemistry results indicated that the prolactin receptor is expressed in the MPG. While solid-phase immunodetection revealed a band with relative mobility of 75 kDa and another similar to 100 KDa. This suggests the existence of the short and long isoforms. We conclude that MPG neurons express the prolactin receptor and that its effects probably are mediated by these two isoforms.
Keywords: prolactin; prolactin receptor; major pelvic ganglion; peripheral nervous system.
Palabras clave
Referencias
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DOI: https://doi.org/10.25009/eb.v16i41.2642
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